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Yields of Spiroiminodihydantoin and 8-OxodG by Various Enzymatic Digestion Methods
| Enzymes | DNA digestion yield | 8-oxodG /106 bases | Spiroiminodi-hydantoin/106 bases |
|---|---|---|---|
| NP1/acid phosphatase | 83 ± 1.5% | 4 ± 0.7 | 10 ± 0.4 |
| NP1/AP (12 h) | 87 ± 0.3% | 15 ± 1.8 | 9 ± 0.6 |
| NP1/AP (24 h) | 90 ± 1% | 15 ± 2.0 | 9 ± 0.7 |
| phosphodiesterase I (5’-exo)/AP | 71 ± 8% | 12 ± 1.7 | 8 ± 0.8 |
| phohphodiesterase II (3’-exo)/AP | 36 ± 4% | 14 ± 1.9 | 10.8 ± 1.2 |
AP = alkaline phosphatase, NP1= nuclease P1
