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. 2008 Mar;3(1):105–115. doi: 10.2147/ijn.s2553

Figure 4.

Figure 4

Light microscopic images of double staining results of patient samples cultured under CNP culture conditions. (A) IIFS of cultured patient samples showed positive fluorescence signal using monoclonal antibody 8D10 when imaged with the green bandpass emission filter, (B) Negative results of Hoechst dye of sample A imaged with the blue bandpass emission filter. (C) IIFS of negative bacterial control (nonpathogenic E. coli strain HB101) showed no fluorescence signal using CNP-specific monoclonal antibody when imaged with green bandpass emission filter. (D) Positive results of Hoechst dye of sample C imaged with the blue bandpass emission filter.