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Effect of bafilomycin A1 on the gene transfection in 293 T cells. WSC/DNA complex was prepared at charge ratio 10 and the amount of PEI used was 0.5 Ag. Different amounts of bafilomycin A1 diluted in DMSO were put into the wells. After 10 min incubation period, transfection solutions were added into the wells. The cells were incubated in the transfection solution for 4 h and then in the growth medium for 48 h (Adapted from Kim et al 2005).
