Figure 1.

Fos protein expression and PMD events apparently occur within the same discrete subpopulation of cells in the injured monolayer, namely those immediately lining the denudation zone. (A) Many (arrowheads indicate two examples), but not all, BAE cells lining the scratch site (green steak) are labeled in their cytosol with the PMD marker, FDx. (B) In a paired image, this same general subpopulation (arrowheads) is seen to have up-regulated fos protein expression, as indicated by the increased immunostaining of nuclei with a fos antibody. This monolayer was fixed 3 h after injury by scratching of the plastic substratum with a sharp implement in the presence of FDx and then immunostained and photographed.