Fig. 1.

Decreased p27 protein expression levels in human uterine leiomyoma tissue.Total protein was isolated from uterine leiomyoma and normal myometrial tissues. Nine pairs of tumor and normal tissues; i.e., each pair of samples was obtained from same patient - M; uterine leiomyoma, N; normal myometrium, 5 of the tissue samples belonged to the proliferative phase (#1 to #5) and the rest to the secretory phase (#6 to #9). Fifty micrograms of the total isolated protein were resolved by SDS-polyacrylamide gel electrophoresis and electroblotted onto PVDF membranes. The blot was incubated with antibody against p27. Reactive bands were visualized with an ECL labeling and detection system. β-tubulin was used as the loading control. Densitometric analysis was also done to the western blots to determine the relative protein expression levels.