Fig. 2.

Decreased p27 protein expression levels in cultured human uterine leiomyoma tissue. Total protein was isolated from cultured, well-grown, confluent uterine leiomyoma and normal myometrium tissue samples (3 pairs of tumor and normal tissue, obtained from same patient - M; uterine leiomyoma, N; normal myometrium, 2 of the cultured cells were from proliferative phase tissue samples (#1 and #2) and #3 was cultured cells of the secretory phase tissue sample). Fifty micrograms of the total isolated protein were resolved by SDS-polyacrylamide gel electrophoresis and electroblotted onto PVDF membranes. The blot was incubated with antibody against p27. Reactive bands were visualized with an ECL labeling and detection system. β-tubulin was used as the loading control. Densitometric analysis was also done to the Western blots to determine the relative protein expression levels.