Fig. 4.

Decreased p27 mRNA levels in human uterine leiomyoma tissue. Total RNA was isolated from uterine leiomyoma and normal myometrial tissues (4 pairs of tumor and normal tissues; i.e., each pair of samples was obtained from same patient; tissue samples #1 and #2 were isolated at the proliferative phase and tissue samples #3 and #4 were isolated at the secretory phases of the menstrual cycle). cDNA templates for RT were prepared from the total RNA extracted. The level of p27 mRNA was determined by quantitative real-time PCR. Relative p27 mRNA levels (normalized to GAPDH) in paired samples represented as M; uterine leiomyoma, N; normal myometrium. ^*, p<0.05; ^**, p<0.01.