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Effect of Ad.p27 transfection on uterine leiomyoma cells. Cultured uterine leiomyoma cells were transfected with Ad.null and Ad.p27 recombinant, non-replicating adenoviral vectors. Twenty-four hours after transfection, immunoblotting was performed to detect the expression levels of p27, p21, Rb, cdk2 and cdk4. β-tubulin was used as the loading control. Transfection of Ad.p27 onto uterine leiomyoma cells caused increased expression levels of p27 and dephosphorylation of Rb.
