Figure 1.

Protein pattern of yeast lipid particles grown in the presence or absence of the squalene epoxidase inhibitor terbinafine. Wild-type yeast cells were grown in the presence (30 μg/ml) or absence of terbinafine as described in MATERIALS AND METHODS. Lipid particles were prepared according to Leber et al. (1994), and 20 μg of total protein were applied to each lane of a 12.5% SDS gel and stained with Coomassie brilliant blue after electrophoretic separation. Lanes 1 and 8, molecular weight standards; lane 2, yeast homogenate (without terbinafine); lane 3, vacuoles (without terbinafine); lane 4, lipid particles (without terbinafine); lane 5, lipid particles (with terbinafine); lane 6, vacuoles (with terbinafine); lane 7, homogenate (with terbinafine).