Table 2.
Distribution of components between the endoplasmic reticulum and lipid particles
| Marker | Enrichment (fold)
|
Relative recovery (%)a
|
||
|---|---|---|---|---|
| Endoplasmic reticulum | Lipid particles | Endoplasmic reticulum | Lipid particles | |
| Sec61pb | 3.9 ± 0.5 | 4.2 ± 0.6 | 98 | 2 |
| BiPb | 1.8 ± 0.3 | 2.7 ± 0.4 | 97 | 3 |
| Steryl estersc | 1.1 ± <0.1 | 860 ± 43 | 6 | 94 |
| Triacylglycerolsc | 1.9 ± 0.2 | 740 ± 59 | 1 | 99 |
| Erg6pb | 2.5 ± 0.3 | 700 ± 91 | 20 | 80 |
| Erg1pb | 4.0 ± 0.5 | 100 ± 12 | 62 | 38 |
Distribution of components was determined based on the relative recovery of components in isolated organelles (see Zinser and Daum, 1995) from cells harvested in the late logarithmic growth phase. Data are mean values from three independent experiments.
Equivalent amounts of protein of each fraction were separated by SDS-PAGE and subjected to Western blot analysis using the respective antiserum. The intensity of the immunological signal in the homogenate was set at 1, and the intensities of signals measured in the endoplasmic reticulum and lipid particles were set in relation. Data were obtained from at least three independent experiments.
Amount of triacylglycerols and steryl esters (mg) per mg of protein in the homogenate was set at 1, and the enrichment in the respective fraction was calculated from the corresponding values. Data were obtained from three independent experiments.