Table 3.
Enzymatic activity of squalene epoxidase in yeast microsomes and lipid particles
| Yeast strain | Subcellular fraction | Specific activity (pmol per min per mg) |
|---|---|---|
| X2180-1A | Microsomes | 48.1 |
| X2180-1A | Lipid particles | ND |
| X2180-1Aa | Lipid particles X2180-1A + microsomes X2180-1A | 44.3 |
| W303 | Microsomes | 14.4 |
| W303 [pAF22] | Microsomes | 75.4 |
| W303 [pAF22] | Lipid particles | ND |
| KLO (anaerobic growth) | Microsomes | 49.2 |
| KLN (anaerobic growth) | Microsomes | ND |
| W303 [pAF22] + KLNa | Lipid particles W303 [pAF22] + microsomes KLN | 25.3 |
Results are mean values of at least three independent measurements (SD ± 10%). ND, not detectable.
a
In mixing experiments, the ratio of lipid particle proteins to microsomal proteins was 1:10.