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. Author manuscript; available in PMC: 2008 Oct 1.
Published in final edited form as: Biotechniques. 2008 Apr;44(5):655–662. doi: 10.2144/000112797

Figure 1. Detecting protein-protein interactions with the Yeast Two-Hybrid system.

Figure 1

Protein-protein interactions are detected by the mating of two haploid complementary yeast strains each expressing a distinct expression plasmid. The first strain expresses a protein prey fused to a transcription activation domain (AD). The second strain expresses a protein bait fused to a DNA-binding domain (DBD) that binds to its cognate binding site, usually upstream of a reporter gene. If there is interaction between bait and prey, the AD is brought into the proximity of the DBD and causes transcriptional activation of the reporter gene leading to selection.