Figure 2.

The role of Src, ERK and PKC in SDF1α-stimulated Jurkat cell migration. (A) Jurkat cells transfected with a control siRNA (siCT) or RACK1 siRNA were pretreated with or without (control) the indicated inhibitors. The transwell migration of Jurkat cells was then induced with or without (basal) 1 nM SDF1α. *p < 0.05, significance versus siCT. (B and C) Effects of the inhibitors on Src (B) and ERK (C) activation. Jurkat cells were pretreated with or without (CT) Src inhibitor I (Src Inh I) or MAPK inhibitor (PD98059) and then stimulated with SDF1α (20 nM for Src and 50 nM for ERKs) for 15 and 2 min, respectively. The activation of Src and ERKs was detected by the indicated specific antibodies against phosphorylated proteins.