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. 2008 Sep;19(9):3909–3922. doi: 10.1091/mbc.E08-04-0433

Figure 5.

Figure 5.

RACK1 specifically regulates SDF1α-stimulated PI3K and PKCζ activities in Jurkat cells. (A–C) AKT phosphorylation at serine 473 in Jurkat cells. Cells were transiently transfected with control (CT) or RACK1 siRNA (A and C), or stably transfected with a control vector, RACK1, or BD1–2 (B) and stimulated with SDF1α (20 nM; A and B) for the indicated times or stimulated with OKT3 antibody at the indicated concentrations for 10 min (C). Representative Western blots are shown on the left panel, and quantitative analysis of data from at least three independent experiments and expressed as percentage increase over the basal in control cells is shown on the right. *p < 0.05, significant difference versus control. (D) RACK1 regulates PKCζ activity. Control (CT) or RACK1 siRNA-transfected Jurkat cells were pretreated in the absence (SDF1α and chelerythrine) or presence of LY294002 (50 μM) and stimulated with SDF1α (50 nM) for 5 min. PKCζ was then immunoprecipitated and assayed for its activity in the presence or absence of chelerythrine (10 μM). *p < 0.05, significance versus control siRNA-treated cells.