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. 2008 Sep;19(9):3638–3651. doi: 10.1091/mbc.E07-12-1272

Figure 4.

Figure 4.

Dck1 is required for matrix-induced filamentous growth. (A) Dck1 but not Dck2 is required for filamentous growth in embedded medium. Wild-type cells (PY82), rac1Δ/rac1Δ cells (PY191), together with homozygous insertion mutant strains dck2::Tn7- UAU1/dck2::Tn7-URA3 (PY284), and dck1::Tn7-UAU1/ dck1::Tn7-URA3 (PY286) were embedded in YEPS (as described in Figure 1). Similar results were observed in four independent experiments. Bar, 1 mm. (B) Dck1 is not required for serum-dependent filamentous growth. Top, wild-type cells (PY82), dck1Δ/dck1Δ cells (PY706) and dck1Δ/dck1Δ PDCK1DCK1 cells (PY710) were embedded in YEPS as described in Figure 1. Similar results were observed in five independent experiments. Bar, 0.5 mm. Bottom, exponentially growing cells were spotted in serial dilution on YEPD FCS and after 5 d, images were taken. Similar results were observed in three independent experiments. Bar, 1 mm.