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. 2008 Sep;19(9):3638–3651. doi: 10.1091/mbc.E07-12-1272

Figure 5.

Figure 5.

CZF1 and RAC1 do not function in the same pathway required for embedded filamentous growth. (A) Overexpression of Czf1 does not restore the embedded filamentous growth defect of rac1 mutant cells. rac1Δ/rac1Δ (PY191) cells, rac1Δ/rac1Δ PADHRAC1 (PY309) cells and rac1Δ/rac1Δ PADHCZF1 (PY456) cells were embedded in YEPS and imaged as described in Figure 1. Similar results were observed in three independent experiments. Bar, 1 mm. (B) Overexpression of constitutively active Rac1 does not restore the embedded filamentous growth defect of czf1 mutant cells. czf1Δ/czf1Δ (PY901) cells, czf1Δ/czf1Δ PADHCZF1 (PY904) cells and czf1Δ/czf1Δ PADHRAC1[Q61L] (PY910) cells were embedded in YEPS and imaged as described in Figure 1. Similar results were observed in three independent experiments. Bar, 1 mm. (C) Rac1[Q61L] protein is overexpressed compared with endogenous Rac1. Extracts of exponentially growing cells indicated in Figure 5B were analyzed by SDS-PAGE, followed by immunoblotting and probing with anti-Rac1 and anti-Cdc11 sera.