Figure 7.

Dck1 and Cdc24 do not have overlapping functions. (A) Overexpression of Cdc24 does not complement the embedded matrix-dependent filamentous growth defect of dck1 mutant cells. dck1Δ/dck1Δ cells (PY706), dck1Δ/dck1Δ PADHCDC24GFP (PY785) cells, cdc24Δ/PMETCDC24 (PY92) cells, and cdc24Δ/PMETCDC24 PADHDCK1 (PY787) cells were embedded in YEPS and imaged as described in Figure 1. Similar results were observed in two independent experiments. Bar, 1 mm. (B) Overexpression of Dck1 does not complement serum dependent filamentous growth defect of cdc24 mutant cells. Exponentially growing cells were added to equal volume of fetal calf serum and incubated at 37°C for 3 h. Images of fixed cells were taken. Bar, 10 μm. Similar results were observed in two independent experiments.