Figure 1.

Effect of 27nt RNA duplex on histone acetylation binding with the eNOS core promoter −238/−13 and intron 4 regions. ChIP assay was performed with anti-acetylated H3 or H4 antibodies in HAECs treated with either siLuc small RNA (negative control) or 27nt RNA. HeLa cells were treated as a control. The eluted DNA was subjected to PCR for the eNOS promoter region (left) and the 27nt repeats in eNOS intron 4.