Figure 2.

Effect of nuclear actin overexpression on acetylation status of histones interacting with 27nt RNA duplex and eNOS promoter and intron 4 regions. ChIP assay shows that nuclear actin overexpression offset the influence of 27nt small RNA at the eNOS core promoter at −238/−13 region and the 27nt repeat region of the intron 4 in HAECs. Chromatin was prepared from HAECs transfected with GFP/actin plasmids and then treated with or without 27nt small RNA. These results were obtained in the same condition as for the ChIP assay described in Figure 1.