Table 1.

ChIP assay primer sets for eNOS gene enhancer, promoter, and the 27nt repeats region of intron 4

Primer pair	Sequence	Amplicon location
En-4917S	5′-CTGTGCTGGGAAGGGAAGCCG-3′	Enhancer
En-4626AS	5′-GCACTCAGCATGAGTTACATCC-3′	4817 to −4626
Pr-1421S	5′-CTGTCTCAGGAGGACGACAAGGAG-3′	Promoter
Pr-1304AS	5′-GGATCCAGCCCCTACTTTTC-3′	1421 to −1304
Pr-891S	5′-TCAAGTGCCTGGAGAGTGCTGG-3′	Promoter
Pr-796AS	5′-GAAGAGCTTGATGCCCTGGTG G-3′	891 to −796
Pr-483S	5′-ACCAGGGGGTCATAAAGGTC-3′	Promoter
Pr-312AS	5′-GGGGAGGTGAAGGAGAGA-3′	483 to −312
Pr-238S	5′-AGCCCTGGCCTTTTCCTTAG-3′	Promoter
Pr-13AS	5′-GCTCCCACTTATCAGCCTCA-3′	−238 to −13
27Re-5318S	5′-AGTACTGGAGGGGAAAGAAGT-3′	27nt repeat region
27Re-5464AS	5′-AGTACTGAAGCTCCGGTCAGC-3′	+5318 to +5464