Table 3.
A summary of the results obtained for Ddi1 and the Ddi1 mutants
| Name | Modification (doublet) | Ddi1 homodimerization | Sso1-binding | pds1-128suppression | Nuclear enrichment |
|---|---|---|---|---|---|
| Ddi1 | Yes | +++ | +++ | +++ | +++ |
| Ddi11–75 | No | NTa | NT | NT | + |
| Ddi11–163 | No | − | − | NT | + |
| Ddi11–326 | No | +++ | +++ | NT | + |
| Ddi11–389 | Yes | +++ | +++ | + | + |
| Ddi1D220A | Yes | +++ | NT | +++ | +++ |
| Ddi178–428 | Yes | +++ | +++ | +/− | + |
| Ddi1Δ202–298 | Yes | − | +++ | ++ | +++ |
| Ddi1Δ323–344 | Partial | +++ | +++ | +++ | +++b |
| Ddi178–326 | No | +++ | − | − | + |
| Ddi1Δ323–390 | No | NT | − | NT | +++ |
| Ddi1Δ344–390 | No | NT | − | NT | +++b |
| Ddi1C300A | Yes | NT | +++ | NT | +++c |
| Ddi1344–395 | No | NT | + | NT | + |
| Ddi1S341A | Yes | NT | +++ | NT | NT |
| Ddi1T346A | No | NT | +++ | NT | NT |
| Ddi1T348A | Partial | NT | +/− | NT | NT |
| Vector control | NT | − | − | − | NT |
| GFP control | NT | NT | NT | NT | + |
| Ddi1 region responsible: | Amino acids 344–390, T346 and T348 | RVP | Amino acids 344–395, T348 | UBL and UBA (RVP and D220) | UBL and UBA |
a NT, not tested.
b Intranuclear accumulation of GFP-Ddi1.
c Intranuclear and cytoplasmic accumulation of GFP-Ddi1.