Skip to main content
. 1999 May;10(5):1309–1323. doi: 10.1091/mbc.10.5.1309

Figure 4.

Figure 4

Representative cell trajectories and motion statistics for parent strain and mutant cells. (A, C, E, and G) Trajectories from a representative tight mound from each strain. Arrowheads represent the location of a cell, and lines connect a cell’s position at successive time points. Time increases in the direction of the arrowheads from black to white. (B, D, F, and H) Schematics summarizing measurements of 3D motion for tight mounds in each strain. See MATERIALS AND METHODS for a definition of each motile category. Motion percentages were determined for each mound and then averaged over the set of mounds in each strain. Each symbol in the schematic reflects a 10% representation of that type of motion in the measured data. Exact percentages are given to the right. (A) Parent strain (JH10). Rotational motion is prominent. Arrowheads represent time points 5 min apart. (B) Average statistics for eight mounds of the parent strain calculated from a total of 400 cell trajectories. (C) MHC− (HS1). Jiggling motion is prominent, and arrowheads representing cell position at each time point are largely superimposed. Time points are 2.3 min apart. (D) Average statistics for 11 mounds of the MHC− calculated from a total of 439 cell trajectories. (E) 3xAsp. Jiggling motion is prominent, and arrowheads representing cell position at each time point are largely superimposed. Time points are 2.5 min apart. (F) Average statistics for four mounds of 3xAsp calculated from a total of 233 cells. (G) RLC−. Random and some rotational motion is evident. Arrowheads represent time points 5 min apart. (H) Average statistics for 22 mounds of RLC− calculated from a total of 1906 cells. Bar, 30 μm.