Figure 6.

NPC and NE dynamics in dividing larval neuroblasts. (A) Selected frames of wide-field time-lapse acquisition of dividing larval neuroblasts expressing mRFP-Nup107 and ketel^GFP. Two representative series describing the prophase to metaphase (top) and the metaphase to telophase stages (bottom) are shown (see also Supplemental Movies 7 and 8). Note that ketel^GFP is released from the prophase NE earlier than mRFP-Nup107 (top, 3:30 and 4:00 min). Both proteins are enriched within the area defined by the spindle envelope in early metaphase, and a fraction of ketel^GFP but not mRFP-Nup107 persists within the spindle area in anaphase (brackets). Note that at these stages, both Nup107 and ketel^GFP are always excluded from the chromatin area (arrowheads). The proteins are recruited simultaneously to the reforming nuclear envelope in early telophase (arrows). (B) Selected frames of wide-field time-lapse acquisition of a dividing larval neuroblast expressing mRFP-Nup107 and GFP-Lamin C. Note the persistence of the GFP-Lamin C staining at the spindle envelope until late anaphase (arrowheads). Arrows point to the reforming NE and NPCs in telophase. Bars, 5 μm. Time is in minutes:seconds.