Figure 4.

LC10 is missing in mutant axonemes lacking outer dynein arms. Axonemes were prepared from wild-type Chlamydomonas and from mutant strains lacking various axonemal components. After electrophoresis, samples were stained with Coomassie blue (top panels) or blotted and probed with CT247 (bottom panels). LC10 is completely missing in strains lacking outer dynein arms (oda1, oda2, oda6, oda9, pf28pf30ssh1) and in the oda12-1 mutant that is null for both LC2 and LC10. However, this protein is present in strains lacking other substructures such as the inner arms (ida1 and ida4) and radial spokes (pf14) or only segments of the outer arm (oda4-s7 and oda11).