Figure 7.

Dynein stability in cytoplasm. (a) Cytoplasmic extract from wild-type Chlamydomonas was sedimented in a 5–20% sucrose density gradient. Equal volumes of each fraction were electrophoresed in a 5–15% acrylamide gradient gel and stained with Coomassie blue. (b) Immunoblot analysis of cytoplasmic extracts from wild-type, fla14, and oda13 strains. Samples were probed with antibodies 1878A, R5932, R5391, R4928, R4058, CT231, and CT247 to detect IC1, LC1, LC2, LC6, LC8, LC9, and LC10, respectively. (c) Model of the Chlamydomonas outer dynein arm and docking complex illustrating the approximate locations and associations of the various components. LC2, LC6, LC8, LC9, and LC10 all interact with the N-terminal regions of the ICs, whereas LC7a and LC7b are thought to associate near the C-terminal WD-repeat domains. Modified from King and Kamiya (2008).