Figure 8.

DNAL4 is present in motile mammalian cilia and flagella. (a) Immunoblot analysis of recombinant murine DNAL4 and Chlamydomonas LC6, LC8, and LC10. The CT254 antibody recognizes DNAL4 and also exhibits weak cross-reactivity with LC10; in contrast, neither LC6 nor LC8 were detected. (b) Tissue homogenates from murine testis, ovary, and brain were electrophoresed in a 5–15% acrylamide gradient gel and stained with Coomassie blue (top panel) or blotted to nitrocellulose and probed with the CT254 antibody (bottom panel). DNAL4 is especially prominent in testis but was also readily detected in the other tissues. (c) Histological analysis of a section through a bronchus from murine lung stained with hematoxylin to detect nuclei and for DNAL4 reactivity (brown). This protein is localized to the cilia at the apical face of the epithelium lining the lung. Bar, 50 μm. The inset shows an enlargement of the ciliated epithelium. (d) Histological analysis of murine testis sections stained either with CT254 (left panel) or with the preimmune serum (right panel) and counterstained with hematoxylin. DNAL4 is very prominent in sperm flagella and in spermatids.