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. 2008 Sep;19(9):3758–3768. doi: 10.1091/mbc.E08-05-0467

Figure 8.

Figure 8.

Glycosylation of CPY in alg mutant strains expressing LmSTT3 proteins. (A) Δalg3 cells (Y03108; left) and Δalg9 cells (Y01993; right) expressing LmSTT3A, LmSTT3B, LmSTT3D or carrying empty vector (v) were grown, protein extracts were separated by SDS-PAGE, transferred to PVDF membrane, and anti-CPY antiserum was used for protein detection. (B) Wild-type (BY4741), Δalg12 (Y05405), Δstt3 (YG2058), and Δalg12Δstt3 (YG2082) strains carrying empty vector (v) or plasmid expressing LmSTT3D were grown, protein extracts were separated by SDS-PAGE, transferred to nitrocellulose membrane, and anti-CPY antiserum was used for protein detection. The position of fully glycosylated CPY (mCPY) and CPY molecules lacking 1 (−1) or 2 (−2) oligosaccharide chains are indicated. Molecular mass of size marker are given at the left.