Figure 3.

Deubiquitylating enzymes differentially affect Ub-chain length at particular H2B lysine residues. Ubiquitylation of the indicated HA-tagged H2B mutants was measured as described in the legend to Figure 1, except that H2B–HA was expressed from a centromeric plasmid in BY4742. Note also that to detect the effects of deleting UBP8 and/or UBP10, we used a cleavable form of His-Ub in which residue G76 of Ub is intact (Yaglom et al., 1995). Four congenic yeast strains were analyzed: WT (BY4742; lanes 1–3), Δubp8 (FGY5; lanes 4–6), Δubp10 (FGY6; lanes 7–9), and Δubp8/Δubp10 (FGY7; lanes 10–12).