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. 2008 Sep;19(9):3801–3811. doi: 10.1091/mbc.E08-04-0352

Figure 5.

Figure 5.

Role of RhoA in formation of ZA. (A) Effects of Y27632 in formation of ZA. When ZO1(wt)/2(wt) Eph4 cells become confluent and are incubated with Y27632, an inhibitor of Rho-kinase, signals for myosin-2/actin (due to phalloidin-staining) suggest that ZA is disintegrated so that the circumferential ring is separated from prezonula-AJ. (B) Transfection of dominant active RhoA (RhoA-DA). In RhoA-DA–expressing ZO1(ko)/2(kd) Eph4 cells, signals for myosin-2/actin are overlapped and linearly arranged at ZA (arrows). (C and D) Transfection of dominant-active Rac1 (Rac1-DA) or dominant-active Cdc42 (Cdc42-DA). Actin is associated with the linearized but fragmented AJ, prezonula-AJ, with which myosin-2 is not associated. A myosin-2 signal exists with weak actin-signal in the cell periphery. Asterisk (*), probability of integration of myosin-2 into cell-cell AJ to form ZA, deduced from 20 transfectants the immunofluorescence micrographs, are shown in blue letters. Bars, 5 μm.