ImmunoEM localization of GRA5–HA9 and GRA5Δ79–86–HA9 within dense granules of Toxoplasma. (A) GRA5–HA9 (anti-HA11, 18-nm gold particles) was found concentrated within the same population of dense granules as GRA1 (TG17–43, 12-nm gold particles). (B) The deletion mutant GRA5Δ79–86–HA9 (anti-HA11, 18-nm gold particles) was also packaged within the same dense granules as GRA1 (TG17–43, 12-nm gold particles). Bar, 100 nm.