Table 4.
Physiochemical parameters of EcoRII as predicted from the amino acid sequence alignments
| Physicochemical parameter | Value |
| Number of amino acids: | 404 |
| Molecular Weight | 45611 |
| Theoretical PI | 6.10 |
| Total number of negatively charged residues (Asp+Glu) | 52 |
| Total number of positive residues (Arg+Lys) | 43 |
| Atomic composition: | |
| • Carbon(C) | 2053 |
| • Hydrogen(H) | 3205 |
| • Nitroge(N) | 565 |
| • Oxygen(O) | 393 |
| • Sulfur | 10 |
| Total number of atoms: | 6426 |
| Formula: | C2053H3205N565O593S10 |
| Extinction coefficients: | 47120(46870) |
| Estimated half-life(hours) | |
| • (mammalian reticulocytes, in vitro) | 30 hour |
| • (yeast, in vivo) | > 20 hours |
| • (Escherichia coli, in vivo) | > 10 hours |
| Instability index: | 45.04 |
| Aliphatic index: | 97.05 |
| Grand average of hydropathicity (GRAVY) | -0.183 |