Figure 2.

C3 pretreatment blocks LPA-induced relocalization of radixin in NIH3T3 cells. (A) NIH3T3 cells stably expressing HA-radixin were transfected with expression plasmids encoding lacZ and C3 transferase (a and b) or lacZ alone (c and d) and then serum-starved and treated with LPA for 15 min. LacZ (b and d) localization (red) is shown to indicate transfected cells, and HA-radixin localization (green) is shown in panels a and c. C3-transfected cells (arrows in panel a) show a lack of radixin localization into apical protrusions after LPA treatment. Bar, 10 μm. (B) Percentage of cells in lacZ only or lacZ plus C3 transfections displaying relocalization of radixin to apical protrusions after LPA treatment. Results of three independent experiments are shown. One hundred transfected cells were counted for each sample. Errors bars denote SD of the mean.