Figure 4.

Cotransfection with RhoV14 relocalizes moesin, but not merlin, to apical membrane protrusions in R12 cells. Cells were transiently transfected with moesin (a), moesin and RhoAV14 (b and c), moesin and RhoAN19 (d), moesin and RacV12 (e), moesin and CDC42v12 (f), merlin (g), or merlin and RhoAV14 (h). Moesin and merlin were tagged with HA-epitope tag at their C termini. HA-moesin (a–f) or HA-merlin (g and h) immunolocalization using the 12CA5 mAb is shown. Panels b and c represent two different focal planes of the same cell to better illustrate the nature of the apical protrusions. All cells presented here were also shown to be positive for GTPase expression (where applicable) by α-myc immunostaining. These transfections were performed seven independent times, and at least 100 transfected cells were examined for each condition. Cells shown are representative of >90% of the transfected cell population. Bar, 10 μm.