Figure 7.

LPA treatment of NIH3T3 cells induces phosphorylation of radixin within 1 min. Metabolically labeled NIH3T3 cells stably expressing HA-radixin or parental (mock) NIH3T3 cells were serum-starved, then treated with LPA (6 μM) for various timepoints as indicated. Cell lysates were immunoprecipitated with 12CA5 (α-HA) mAb. Radixin is indicated by arrowhead at left. Radixin phosphorylation was increased threefold within 1 min of LPA treatment and remained constant for 15 min. Note background band (*) whose orthophosphate content was not apparently modulated by LPA. Molecular size standards are indicated at right. The data shown are representative of four independent experiments.