Table 1.
S. Typhimurium mutants that exhibited different type 1 fimbrial phenotypes than the parental strain
| Category | Mutant | Phenotype | Genea | Function |
| (agar, broth) | ||||
| Fimbrial biosynthesis and regulation | K2 | -, - | fimZ | Positive regulator of type 1 fimbriae major subunit gene fimA |
| K14 | -, - | fimY | Positive regulator of type 1 fimbriae major subunit gene fimA | |
| K40 | -, - | fimA | Major subunit of type 1 fimbriae | |
| K66 | -, - | fimD | Fimbrial usher protein of FimA | |
| K75 | -, - | fimC | Fimbrial chaperone protein of FimA | |
| K44 | -, - | fimH | Adhesin of type 1 fimbriae | |
| K70 | +, + | lpfB | Fimbrial chaperone protein of long polar fimbriae | |
| K61 | +, + | pefI | Regulator of plasmid-encoded fimbriae | |
| K23 | +, + | stbC | Fimbrial usher protein of Stb fimbriae | |
| Putative cytoplasmic protein | K1 | +, + | STM1078 | Putative cytoplasmic protein |
| K28 | +, + | STM4529 | Putative cytoplasmic protein | |
| Global regulator | K46 | -, - | lrp | Leucine-responsive regulatory protein |
| K15 | -, - | hupA | Histone-like DNA binding protein | |
| Membrane associated protein | K17, | +, + | yhjV | Putative transporter protein |
| K39 | -, - | STM1128 | Putative sodium/glucose cotransporter | |
| K45 | -, - | yihO | Putative glycoside-pentoside-hexuronide (GPH) family transport protein | |
| K50 | +, + | STM2532 | Putative inner membrane lipoprotein | |
| K52 | +, + | STM2486 | Putative inner membrane protein | |
| K54 | -, - | yjiH | Putative inner membrane protein | |
| K72 | +, + | mgtB | Mg2+ transporter protein | |
| K30 | +, + | narK | Nitrate/nitrite transporter protein | |
| Cell envelope associated protein | K60 | +, + | ytfB | Putative cell envelope opacity-associated protein A |
| Periplasmic protein | K69 | +, + | ydbH | Putative periplasmic protein |
| Ribosome modulation factor | K56 | -, - | rmf | Ribosome modulation factor |
| Type III secretion system | K55 | -, - | ssaB | Effector protein of type III secretion system |
| K59 | +, + | invB | Chaperone protein for SopE/SopE2 | |
| K63 | -, - | ssaV | Apparatus protein for intracellular trafficking and secretion | |
| Prophage-derived protein | K11 | +, + | STM4200 | Putative phage tail protein H |
| K33 | +, + | sseI | Gifsy-2 prophage putative type III secreted effector protein | |
| K19 | -, - | STM1041 | Gifsy-2 prophage probable minor tail protein | |
| Sensor protein | K51 | +, + | ssrA | Sensor component of type III secretion system |
| K58 | +, + | yehU | Sensor component | |
| Transcription termination factor | K73 | -, - | nusA | Transcription termination factor |
| Enzymes | K3 | -, - | cafA | Endoribonuclease |
| K5 | +, + | res | Subunit of type III restriction-modification enzyme | |
| K8 | +, + | STM4467 | Putative arginine deiminase | |
| K16 | +, + | STM1627 | Alcohol dehydrogenase class III | |
| K31 | +, + | STM2446 | Putative iron-dependent peroxidase | |
| K41 | -, - | nuoE | NADH dehydrogenase I chain E | |
| K37 | +, + | yafA | Putative hydrolase | |
| K43 | -, - | miaA | Isopentenylpyrophosphate transferase | |
| K38 | -, - | prC | C-terminal protease for penicillin binding protein | |
| K47 | -, - | atpH | Membrane-bound ATP synthase | |
| K48 | +, + | ubiB | NAD(P)H-flavin reductase | |
| K49 | -, - | ydiF | Putative acetyl-CoA: acetoacetyl-CoA transferase | |
| K53 | +, + | tdK | Thymidine kinase | |
| K65 | +, + | pps | Phosphoenolpyruvate synthase | |
| K57 | +, + | ybjX | Putative enzyme with unknown function | |
| K67 | +, + | pncA | Nicotinamidase/pyrazinamidase | |
| K68 | +, + | rfaB | Lipopolysaccharide 1,6-galactosyltransferase | |
| K71 | +, + | xapB | Xanthosine permease | |
| K64 | +, + | STM1940 | Putative cell wall-associated hydrolase | |
| K74 | +, + | cbiA | Cobyrinic acid a, c-diamide synthase | |
| K42 | -, - | aroA | 3-enolpyruvylshikimate-5-phosphate synthetase |
a: The gene that was disrupted by the transposon insertion is listed. The annotation of S. Typhimurium LT2 genome is used if the transposon insertion was present in a gene which is uncharacterized and unnamed.