Figure 10.

Effect of antioxidant treatment on eIF2α phosphorylation following cigarette smoke exposure. A549 cells were exposed to air (mock treatment) or 2R4F cigarette smoke for 20 minutes with 35 cc puffs diluted in 250 cc air, with or without concurrent treatment with NAC or GSH (at 5 and 25 mM concentrations), after which the cells were placed in fresh medium and returned to the incubator for the time periods specified. Western blots of whole cell lysates were probed with antibodies to phosphorylated eIF2α and GAPDH.