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. Author manuscript; available in PMC: 2008 Dec 25.
Published in final edited form as: Biochemistry. 2007 Dec 4;46(51):15198–15207. doi: 10.1021/bi701891t

Figure 3.

Figure 3

GALM mRNA is induced by RA, and completely inhibited in cells pretreated with actinomycin D, but not with cycloheximide. A, THP-1 cells were treated with either RA (20 nM), actinomycin D (20 nM), RA + actinomycin D, or vehicle (control) for 24 h. B. THP-1 cells were treated with either RA (20 nM), cycloheximide (20 nM), RA+ cycloheximide, or vehicle (control) for 24 h. After incubation, cells were harvested and GALM and GAPDH mRNAwas measured by real-time RT-PCR as described in Materials and Methods. Data are means (with control set to 1) ± SEM, n=3/treatment; * denotes different from control, P<0.05.