Figure 5.
Time dependent up-regulation of galactose-related genes in THP-1 cells treated with RA. THP-1 cells were treated with RA (20 nM) at t=0 and again after overnight incubation (shown by black triangles); microarray analysis was conducted (as described in Fig. 1B and Materials and Methods). Median signal intensity for the arrays is 0 on a log(2) scale and the Y-axis displays the mean ± SEM of the log(2) values of the array signal intensities at each time. Bars or points that do not share a superscript letter differed significantly (P values as shown). Genes illustrated are A, B4GALT5 (Entrez Gene ID 9334; Feature IDs 221484_at, and 221485_at); B, ST3GAL3 (Entrez Gene ID 6487; Feature ID 1555702_a_at); C, ST6GALNAC5 (Entrez Gene ID 81849; Feature ID 220979_s_at); and D, GALNAC4S-6ST (Entrez Gene ID 51363; Feature ID 203066_at).