TABLE 2.
Γ | G(OH●)a | Gdir(fbr)b | G(dsb)c | Gdir(ssb)d | m(ssb)e | Gdir(ssb)/G(dsb) | GdRib(fr)f | G(diff)g |
---|---|---|---|---|---|---|---|---|
2.5 ± 0.2 | 0 | 134 ± 4 | 4.8 ± 0.9 | 124 ± 4 | 1.4 ± 0.2 | 26 ± 5 | 33 ± 5 | 91 ± 4 |
22.5 ± 1.1 | 23 | >189 ± 21 | 3.5 ± 0.5 | >182 ± 21 | >2.8 ± 0.5 | >52 ± 10 | 79 ± 12 | >103 ± 21 |
<212 ± 21 | <205 ± 21 | <3.1 ± 0.5 | <59 ± 10 | <126 ± 21 |
A yield of 70 nmol/J (5) was applied to the water target mass, which consists of the water lying beyond the hole transfer layer: Γ-10 = target mass = 12.5 waters/nucleotide for Γ = 10.
Gdir(fbr) ≥ G(fbr) − G(●OH) and ≤G(fbr).
Values from ref. (12).
Gdir(ssb) = G(fbr) − 2 × G(dsb), assuming each SSB of the DSB gives a free base release.
SSB multiplicity = m(ssb) = Gdir(ssb)/G(sc-loss).
GdRib(fr) values were reported in Table I of ref. (13).
Shortfall = G(diff) = Gdir(ssb) − GdRib(fr).