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. 2008 Sep;173(3):762–772. doi: 10.2353/ajpath.2008.080175

Figure 2.

Figure 2

Generation of the regulatable transgenic mouse model rTg3696AB. A: Mice expressing both Tg3696A and Tg3696B were identified by Southern blot. B: Activator mice expressing the forebrain-specific neuronal CAMKII promoter were crossed with responder mice expressing both APPNLI and TauP301L transgenes. C: Specific transgene expression in the forebrain was confirmed by in situ hybridization in rTg3696AB mice. D and E: Western blots confirmed transgenic protein expression in the forebrain of 11-month rTg3696AB mice. APPNLI was detected using 6E10 and tau was detected after incubation with Tau-5 primary antibody. APP and tau transgene expression is compared to Tg2576 (APP) and Tg4510 (Tau) as positive controls, respectively.