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. 2008 Sep;173(3):762–772. doi: 10.2353/ajpath.2008.080175

Figure 3.

Figure 3

APP expression and Aβ plaque development in rTg3696AΒ mice. A–C: IHC studies with 4G8 confirm strong APP expression in the forebrain hippocampus of 4-month rTg3696AB+/+ mice (A, hippocampus; B, CA1; C, frontal cortex) compared to age-matched nontransgenic littermates (D–F). Positive somatic labeling was shown to represent transgenic APP using antibody 22C11 (G and I, CA1; H and J, frontal cortex). Aβ plaques (arrow) were first observed in the frontal cortex at 4 months of age, as detected by 4G8 (K), Aβx-40 (L), and Aβx-42 (M). Human cortical tissue from an AD patient was processed in parallel as a positive control for detection of Aβ plaques using 4G8 (N). Serial sections of 24-month Tg2576 brain were used as positive controls for antibodies Aβx-40 (O) and Aβx-42 (P). Original magnifications: ×5 (A, D); ×10 (K–P); ×40 (B, C, E–J).