Figure 1.
The myogenic ability of AMMCs is greater than primary myoblast cultures. Fluorescent staining for γ-sarcoglycan protein revealed large clusters of donor-derived myofibers 1 month after transplantation in Sgcd-null muscles injected once with 105 AMMCs. In contrast, injection of cultured myoblasts resulted in scattered, isolated sarcoglycan-positive muscle fibers. Muscles injected with AMMCs had 35-fold more sarcoglycan-positive myofibers than those injected with cultured myoblasts. Sarcoglycan-positive fibers were scored on at least 140 sections per recipient muscle. Shown are the average values from the section with the most sarcoglycan-positive fibers from each recipient. Original magnifications, ×100.