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Multiple injections of AMMCs increase sarcoglycan expression. Sarcoglycan-positive fibers were quantified in tibialis anterior muscles 1 month after receiving the first of one, two, or three injections of 105 AMMCs. Three injections increased the number of sarcoglycan-positive fibers (P = 0.04, one injection compared to three injections) indicating that increased exposure to donor-derived cells and sarcoglycan expression did not adversely affect engraftment and survival.
