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. 2008 Sep;173(3):824–834. doi: 10.2353/ajpath.2008.071005

Figure 7.

Figure 7

ER, progesterone receptor, and cyclin D1 expression in tissues and tumors, measured by QPCR (A–C) and Western analysis (D). ER mRNA expression in whole postpubertal mammary glands and mammary tumors from wild-type (WT) and BK5.IGF-1 transgenic (Tg) mice (A). Progesterone receptor mRNA expression in tumors (average of 10 different samples) from untreated wild-type and transgenic mice and in tumors from one wild-type and one transgenic mouse 6 hours after intraperitoneal injection of 17β-estradiol (20 μg/kg) (B). Cyclin D1 mRNA expression in tumors from wild-type and BK5.IGF-1 transgenic mice (C). Numbers of samples analyzed are noted within each bar. All mRNA levels were normalized to the expression of TATA box binding protein mRNA as described in Materials and Methods. Western blot analysis of cyclin D1 protein levels in individual mammary tumors from wild-type and BK5.IGF-1 transgenic mice (D). Blot was reprobed with antibody to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to demonstrate equal loading.