Figure 2.

Comparison of adult and neonatal keratinocytes in the development of normal human skin equivalents. Abdominal (A--E) or foreskin (F--J) human keratinocytes were seeded on DED, and cultured for 4 days submerged, followed by 10 days of culture at the air-liquid interface. Morphology of the skin constructs was studied by H&E staining (A and F), whereas protein expression of CK10 (B and G), CK16 (C and H), hBD-2 (D and I), and SKALP/elafin (E and J) was determined by immunohistochemistry. Note the expression of SKALP/elafin and the presence of parakeratosis in the constructs derived from neonatal foreskin keratinocytes.