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Effect of cytokine stimulation on SKALP/elafin and hBD-2 protein expression in skin equivalents. Human skin equivalents were stimulated (B–D and F–H) or not (A and E) for 4 days with 10 ng/ml of IL-1α (B and F); 10 ng/ml of IL-1α and 5 ng/ml of TNF-α (C and G); or 10 ng/ml of IL-1α, 5 ng/ml of TNF-α, and 5 ng/ml of IL-6 (D and H). SKALP/elafin (A–D) and hBD-2 (E–H) protein expression was determined by immunohistochemistry.
