FIGURE 3.

Inhibition of macroautophagy increases endogenous (A) and human overexpressed (B) WT ASYN levels in PC12 cells. Cells were cultured in the presence of 3-MA (10 mm) or epoxomicin (1 μm) for 14 h. Untreated cells were used as a control (Ctrl). Cell lysates were assessed by Western immunoblotting for ASYN levels. Left, representative immunoblot of ASYN. Right, quantification of endogenous (A) or overexpressed (B) ASYN levels after 3-MA or epx addition, compared with control. Results are expressed as the ratio of OD values to the corresponding controls, and data are presented as mean ± S.E. of six (for 3-MA) or three (B, for epx) independent experiments. (*, p < 0.05; ***, p < 0.001, Student's t test comparing 3-MA or epx with the control).