FIGURE 6.

Antagonistic properties of ASF/SF2 and SC35. GH3 cells were transfected with 500 ng of either SC35 or ASF/SF2 expression vector and increasing amounts (100, 250, 500, and 1000 ng) of the other expression vector. Empty vectors were used to balance each transfection to an equal mass of DNA. GH1 splicing was analyzed by RT-PCR and polyacrylamide gel electrophoresis. The amounts of exon 3 skipped transcripts in each lane were calculated as a percentage of all other transcripts within a single lane. The data are presented as the averages of at least n = 3. The error bars are S.E. A, wild-type GH1. B, A1338G mutant GH1. C, A1338G+Δ6 mutant GH1.