FIGURE 4.
Phosphorothioate analysis of pre-steady-state kinetics of dCTP incorporation opposite N2-BPG by human REV1. REV1 (240 nm) was incubated with 100 nm 24-mer/36-mer primer-template complex in the rapid quenched-flow instrument and mixed with 1 mm dCTP (▪) or (Sp)-dCTPαS (▾) to initiate reactions for the 24-mer/36-N2-BPG-mer. The pre-steady-state rates were determined from the burst equation and are indicated in the figure. The following rates were estimated: dCTP, kp = 0.83 ± 0.29 s–1; (Sp)-dCTPαS, kp = 1.0 ± 0.2 s–1.