TABLE 2.

Steady-state kinetic parameters for one-base incorporation opposite guanine N2 adducts by human REV1

Template	dNTP	Km	kcat	kcat/Km	f(misinsertion ratio)
		μm	s-1	mm-1 s-1
G	C	13 ± 2	0.0120 ± 0.0004	0.92	1
	G	250 ± 50	0.0014 ± 0.0001	0.0056	0.0061
	T	350 ± 60	0.0021 ± 0.0001	0.0060	0.0065
N2-MeG	C	25 ± 2	0.0088 ± 0.0002	0.35	1
	G	170 ± 40	0.00064 ± 0.00004	0.0038	0.011
	T	440 ± 90	0.0015 ± 0.0001	0.0034	0.010
N2-EtG	C	9.4 ± 1.8	0.0046 ± 0.0002	0.49	1
	G	290 ± 90	0.0014 ± 0.0001	0.0048	0.010
	T	400 ± 60	0.0019 ± 0.0001	0.0048	0.010
N2-IbG	C	22 ± 3	0.0124 ± 0.0004	0.56	1
	G	260 ± 80	0.0034 ± 0.0004	0.013	0.023
	T	380 ± 50	0.0049 ± 0.0002	0.013	0.023
N2-BzG	C	6.9 ± 0.6	0.0085 ± 0.0002	1.2	1
	G	180 ± 40	0.0040 ± 0.0003	0.022	0.018
	T	310 ± 70	0.0051 ± 0.0004	0.016	0.013
N2-NaphG	C	4.4 ± 0.2	0.0041 ± 0.0001	0.93	1
	G	220 ± 60	0.0025 ± 0.0002	0.011	0.012
	T	230 ± 30	0.0015 ± 0.0001	0.0065	0.0070
N2-AnthG	C	7.8 ± 1.5	0.0024 ± 0.0001	0.31	1
	G	100 ± 20	0.0014 ± 0.0001	0.014	0.045
	T	320 ± 60	0.0023 ± 0.0002	0.0072	0.023
N2-BPG	C	2.3 ± 0.3	0.002 ± 0.0001	0.87	1
	G	70 ± 10	0.00084 ± 0.00003	0.012	0.014
	T	80 ± 10	0.0011 ± 0.0001	0.014	0.016